Monoclonal Antibodies That Bind to Cell Surface Antigens on Human Embryonal Carcinoma Cells
Barbara Knowles, Davor Solter, and Peter Andrews
Wistar researchers have developed monoclonal antibodies against five cell surface markers that are useful in the identification and characterization of human embryonic stem cells and monitoring their differentiation. Investigation of the function and expression of developmentally regulated cell surface antigens may yield clues to the control of cell differentiation, not only during embryogenesis but also during oncogenesis. These monoclonal antibodies bind to cell surface antigens SSEA-1 (stage-specific mouse embryonic antigen), SSEA-3, SSEA-4, TRA-1-60R, and TRA-1-81.
MC-631 (SSEA-3) hybridomas were produced by fusing SP2/0 mouse myeloma cells with splenocytes from a Fisher rat immunized with 4-8 cell stage mouse embryos.
MC-813-70 (SSEA-4) hybridomas were produced by fusing SP2/0 myeloma cells with splenocytes from a BALB/c mouse immunized with human embryonal carcinoma cell line 2102Ep.
TRA-1-60R and TRA-1-81 hybridomas were produced by fusing SP2/0 myeloma cells with splenocytes from a BALB/c mouse immunized with human embryonal carcinoma cell line 2102Ep.
MC-631 (SSEA-3) and MC813-70 (SSEA-4): early mouse embryos, human teratocarcinoma stem cells, human embryonal stem cells. MC-631, IgM; MC-813-70, IgG3
TRA-1-60R and TRA-1-81: human embryonal carcinoma cell lines. IgM.
Immunohistochemistry, Immunoprecipitation. Identification of human embryonic stem cells. Characterization of embryonal stem cells of human origin to distinguish them from those of murine origin.
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