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Anti-Human Tenascin Monoclonal Antibodies 300-1, 300-3, 300-2, 302-1, 302-9

Tech ID

MAbs 300-1, 300-3, 300-2, 302-1, 302-9 were derived by immunizing BALB/c mice with a high molecular weight fraction of culture supernatants of melanoma cells. Fusion was done with a non-secreting variance of myeloma P3x63Ag8.653.

Tenascin is a large glycoprotein of the extracellular matrix and can be found in adult tissues during wound healing and tumorigenesis. These MAbs define four different epitopes on the human tenascin molecule. Tenascin levels are increased in sera of patients with advanced melanoma, and melanoma cells in culture secrete tenascin constitutively but do not attach to it. Glioma cells are also major producers of tenascin. Radiolabeled anti-tenascin antibodies can be used for radiolocalization and therapy of gliomas.

Key Words
Industrial Relevance: Diagnosis of tenascin in serum; radiolocalization and therapy of gliomas
Applications and Advantages

Immunohistochemistry, western blot, serum detection.