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Imaging Facility

Overview

The imaging of complex cellular structures is used to determine how the temporal and spatial organization of regulatory events within cells, tissues and organisms impacts both normal and pathological processes. The state-of-the-art Imaging Shared Resource provides access to standard and advanced optical imaging systems capable of reaching these goals and offers assistance with advanced image analysis solutions. Researchers may be trained for unassisted use of all core instrumentation, while full-service assistance by facility staff is also available for quantitative or qualitative image capture. The Facility also offers expert technical assistance with experimental design to optimize imaging results, enabling users to get more out of the imaging technology.

Services

  • Brightfield and Fluorescence Widefield Microscopy
  • Spectral Confocal microscopy
  • 2-photon intravital microscopy
  • Advanced quantitative imaging (FRET, FRAP, photobleaching, colocalization)
  • 2D, and 3D, multichannel, multipoint, live-cell, timelapse imaging
  • Small animal, whole body luminescence, fluorescence, and CT imaging
  • Low magnification and photomacrography
  • Personalized training and assistance on all imaging systems
  • Personalized image analysis training and services
  • Automated and facilitated whole slide and plate scanning
  • Assistance with microscopes within individual labs
  • Traditional photographic services

Equipment & Features

  • Upright widefield microscopes (brightfield and fluorescence)
    • Nikon Eclipse NiE motorized, automated upright microscope 
    • Nikon 80i upright microscope
    • Nikon E600 upright microscope
    • Nikon SMZ800 and 1500 stereomicroscopes
  • Inverted widefield microscopes (brightfield and fluorescence)
    • Fully Automated Nikon Eclipse TiE inverted microscope with environmental chamber 
    • Semi-automated Nikon TE300 inverted microscope with environmental chamber
    •  Nikon TE2000 inverted microscope
  • Laser-based instruments (for fixed and live-cell imaging)
    • Leica TCS SP8 X WLL laser scanning confocal microscope with environmental chamber
    • Leica TCS SP5 II laser scanning confocal microscope with environmental chamber
    • Leica TCS SP8 MP 2-photon intravital microscope
  • Other imaging systems
    • Perkin Elmer IVIS SpectrumCT small animal, whole body imaging system
    • Digital SLR camera systems
    • Advanced image analysis workstations

Pricing

For pricing information, visit iLab or contact the managing director.

This facility is supported in part by a Cancer Center Support Grant (CCSG) awarded by the National Cancer Institute (NCI) to The Wistar Institute Cancer Center.

Click here for information on how to acknowledge our Shared Resources in your publications and grants.

Contact Us

The Wistar Institute
Imaging Facility, Room 287
3601 Spruce Street
Philadelphia, PA 19104
215-898-3887

Facility Hours:
8:30 a.m.-5:00 p.m., Monday-Friday

Qing Chen, M.D., Ph.D.
Scientific Director

James Hayden, B.A., R.B.P., F.B.C.A.
Managing Director

Frederick Keeney, B.S.
Wistar Research Assistant