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Imaging Facility

Overview

The imaging of complex cellular structures is used to determine how the temporal and spatial organization of regulatory events within cells, tissues and organisms impacts both normal and pathological processes. The state-of-the-art Imaging Shared Resource provides access to standard and advanced optical imaging systems capable of reaching these goals and offers assistance with advanced image analysis solutions. Researchers may be trained for unassisted use of all core instrumentation, while full-service assistance by facility staff is also available for quantitative or qualitative image capture. The Facility also offers expert technical assistance with experimental design to optimize imaging results, enabling users to get more out of the imaging technology.

Services

  • Brightfield and Fluorescence Widefield Microscopy
  • Spectral Confocal microscopy
  • 2-photon intravital microscopy
  • Advanced quantitative imaging (FRET, FRAP, photobleaching, colocalization)
  • 2D, 3D, multichannel, multipoint, live-cell, timelapse imaging
  • Small animal, whole body luminescence, fluorescence, CT and ultrasound imaging
  • Low magnification and photomacrography
  • Automated whole slide and plate scanning
  • Personalized training and assistance on all imaging systems
  • Personalized image analysis training and services
  • Assistance with microscopes within individual labs
  • Traditional photographic services

Equipment & Features

Upright widefield microscopes (brightfield and fluorescence)
  • Nikon 80i Upright Microscope
  • Nikon Eclipse NiE motorized, automated upright microscope
  • Nikon SMZ800 and 1500 stereomicroscopes
  • Hamamatsu Nanozoomer S60 Whole Slide Scanner
Inverted widefield microscopes (brightfield and fluorescence)
  • Nikon TE2000 inverted microscope
  • Nikon Eclipse TiE automated, inverted microscope with environmental chamber
  • Nikon TE300 semi-automated, inverted microscope with environmental chamber
  • Leica Mica WideFocal Live Cell Microhub
Laser-based instruments (for fixed and live-cell imaging)
  • Leica Stellaris 8 FALCON WLL 3X TauSTED laser scanning confocal microscope
  • Leica Stellaris 8 FALCON WLL laser scanning confocal microscope
  • Leica TCS SP8 X WLL laser scanning spectral confocal microscope
  • Leica TCS SP8 MP-2 photon intravital microscope
    Pre-clinical imaging systems

    • Revvity IVIS SpectrumCT small animal whole body imager
    • Revvity Vega ultrasound system with Image Guided Injection
    • Xstrahl Small Animal Radiation Research Platform (SARRP)
    Other Imaging Systems
    • Traditional digital camera systems (Nikon Z6II)
    • Advanced image analysis workstations
    • Monoprice Vector IIIP 3D printer

    Pricing

    For pricing information, visit iLab or contact the managing director.

    This facility is supported in part by a Cancer Center Support Grant (CCSG) awarded by the National Cancer Institute (NCI) to the Ellen and Ronald Caplan Cancer Center.

    See recording of facility presentation.


    Contact Us

    The Wistar Institute
    Imaging Facility, Room 287
    3601 Spruce Street
    Philadelphia, PA 19104
    215-898-3887

    Directors/Assistants

    Nan Zhang, Ph.D.
    Scientific Director

    James Hayden, BA, RBP, FBCA
    Managing Director

    Fengchong Kong, MS
    Co-Managing Director

    Frederick Keeney, BS
    Assistant Managing Director

    Shang Wu
    Research Assistant II

    Facility Hours

    Monday-Friday
    8:30 am-5:00 pm