Gene Editing Institute

Wistar and the Gene Editing Institute (GEI) at ChristianaCare’s Helen F. Graham Cancer Center & Research Institute maintain a partnership that aims to accelerate breakthrough cancer research in the human genome. 

The Gene Editing Institute is integrated into Wistar’s Shared Resource, which allows its innovative gene editing technologies to be available to research projects at Wistar and to external users. Led by Eric B. Kmiec, Ph.D., the GEI works directly in the design, synthesis and optimization of gene editing tools (CRISPR/Cas9, TALENs, etc.) that can be used across a wide array of biomedical applications. 

SERVICES

Knock-out services: 

• Single gene knockout clonal cell lines 
• Multiplex knockout clonal cell lines 
• Large deletion clonal cell lines 
• Knockout pool 

Knock-in services: 

• Point-mutation clonal cell lines 
• Insertional mutation (epitope tagging, etc) clonal cell lines 
• Large insertional mutation (fluorescent protein fusion, transcriptional termination, etc) clonal cell lines 
• Multiplex knock-in clonal cell lines 

Knock-out library services: 

• Whole human genome knock-out libraries (6 gRNAs per gene) 
• Targeted genome knock-out libraries (6 gRNAs per gene) 

Specialized services: 

• Gene repression through CRISPR/Cas9i 
• Gene activation through CRISPR/Cas9a