Lab In The News
The Campbell Foundation Awards $100,000 Grant to The Wistar Institute
The nonprofit organization, which is dedicated to funding HIV/AIDS research, has awarded the grant to Mohamed Abdel-Mohsen, Ph.D., assistant professor in Wistar's Vaccine & Immunotherapy Center.
The Abdel-Mohsen Laboratory
The Abdel-Mohsen laboratory investigates the role of host-virus interactions in persistence and immunopathogenesis of HIV infection by combining virological, glycobiological, and immune-based basic and translational research. The multidisciplinary studies aim to develop novel approaches to enhance immune function and control/eradicate HIV.
The focus of the work in the lab is based on a combination of approaches including investigating the role of glycan-lectin interactions and altered cell-surface glycosylation in mediating cellular processes central to immune regulation and human diseases; designing multi-omics approaches to study host immune response to HIV infection; and developing and implementing robust and sensitive molecular biology-based assays to measure cellular and tissue HIV reservoirs.
Florent Colomb, Ph.D.
Leila Giron, Ph.D.
Alitzel Greet Anzurez Reyes
Glycoimmunology is an emerging field focused on understanding how immune responses are mediated by glycans (carbohydrates) and their interaction with glycan-binding proteins called lectins. How glycans influence immunological functions is increasingly well understood. In parallel, research in the HIV field is unveiling how the host immune system controls HIV persistence and immunopathogenesis. However, the role played by the host glycosylation machinery in modulating the persistence and immunopathogenesis of HIV has mostly been overlooked, despite its potential for therapeutic applications.
Our laboratory is using several advanced glycomic technologies to investigate the role of the host glycosylation machinery in regulating molecular mechanisms central to HIV infection. We aim to create a new paradigm for discovering novel biomarkers of viral/host interactions and/or glycan-based interactions that can be therapeutically targeted to cure HIV and/or enhance the quality of life for people living with it. We believe that our research has the potential to expand the boundaries of current knowledge about the link between infections, chronic inflammation and the development of chronic diseases. This information will be important not just for HIV, but for other diseases involving inflammation, autoimmune disorders, cancer, and pathogen infections.
Below, we illustrate four areas in which the links between glycan-lectin interactions and immunology, and between immunology and HIV are described. Our laboratory is investigating the links between glycoimmunology and HIV persistence/immunopathogenesis within these areas.
Chronic inflammation has been associated with aberrant IgG glycosylation patterns and is prevalent in HIV+ individuals despite antiretroviral therapy (ART). Sialylated and galactosylated glycans have been associated with anti-inflammatory responses while bisected N-acetylglucosamine (GlcNAc) has been associated with pro-inflammatory responses. HIV infection causes pro-inflammatory changes, e.g., ART-irreversible loss of sialic acid and ART-reversible loss of galactose. Whether the HIV-induced changes in the circulating glycome are linked to chronic inflammation and HIV-associated co-morbidities (such as cardiovascular diseases and neurological impairments) is not clear. Asn = Asparagine.
Antibody-mediated effector functions are significantly affected by changes in IgG glycosylation and are important for preventing and controlling HIV infection. The presence of core fucose reduces antibody-dependent cellular cytotoxicity (ADCC), and the presence of galactose induces ADCC, antibody-dependent cellular phagocytosis (ADCP) and complement-dependent cytotoxicity (CDC). The size of the HIV reservoir, measured using nucleic acid-based methods (CD4+ T cell-associated HIV DNA and RNA), negatively associates with levels of non-fucosylated galactosylated glycans during suppressive ART. However, it is not clear if the documented roles of non-fucosylated galactosylated glycans in promoting ADCC and ADCP impact viral control during ART. C1q = Complement component 1q.
The potential role of the gut glycome in regulating the homeostatic relationship between the host and its gut microbiota during HIV infection. The potential role of the gut glycome in regulating the homeostatic relationship between the host and its gut microbiota, during HIV infection. The degree of glycosylation in the gut directly impacts the ability to maintain functional and healthy intestines. Here we give one example, by illustrating the role of gut fucosylation in the host-microbe interplay. Fucosylated glycans in the gut (left) enhance the beneficial activity of symbionts and improve resistance against colonization by pathogens and pathobionts. In the absence of gut fucosylation (right), beneficial symbionts are weakened and decreased in abundance, and pathogenic bacteria increase, which leads to microbial translocation, inflammation, and breakdown of the epithelial barrier. Fucosylated glycans are only one group out of many glycan structures composing the gut glycome. A change in the gut glycome may alter the distribution of microbial species. Therefore, it is possible that alterations in glycan metabolism may contribute to HIV-mediated intestinal damage, microbial translocation, and chronic inflammation.
Cell-surface glycan-lectin interactions mediate signals that define cellular processes and immunological functions, many of which are central to HIV infection. The specific structure of a glycan allows it to bind to specific glycan-binding proteins called lectins, leading to activation of downstream signaling pathways. These pathways are critical for a variety of cellular processes and immunological functions:
- T cells. Galectin-1 induces T cell apoptosis. Galectin-9 induces T-cell receptor (TCR) signaling, while galectin-3 reduces it. Galectin-3 alters T-cell function through interaction with LAG3 and other immune negative checkpoints. Last, the fucosylation of PD-1 impacts its function.
- NK cells. Siglecs-7 and -9 inhibit NK activity. Galectin-9 impairs NK function/cytotoxicity and cytokine production. Galectin-3 antagonizes NK cell-mediated antitumor immunity
- B cells. Siglec-6 induces B-cell exhaustion. Galectin-1 is a pre-B cell receptor ligand that induces receptor clustering, leading to efficient B cell differentiation. Galectin-9 suppresses B-cell receptor (BCR) signaling.
- T-regs. Galectins-1 and -9 can expand T-regs.
- Myeloid-derived suppressive cells (MDSC). The galectin-9/Tim3 interaction drives the expansion of CD11b+ly6G+ MDSC. Granulocytic MDSCs induce γδ-T cells to produce galectin-1, thus transforming them into immunosuppressive cells. These glycan-lectin interactions represent potential novel targets to enhance immune functionality during HIV infection to either cure HIV or prevent HIV-associated immune dysfunction and the subsequent development of immune dysfunction-associated diseases.
Abdel-Mohsen, M., Kuri-Cervantes, L., Montaner, L.J., et al. “CD32 is expressed on cells with transcriptionally active HIV but does not enrich for HIV DNA in resting T cells.” Sci Transl Med. 2018 Apri 18;10 (437) pii: eaar6759. doi: 10.1126/scitranslmed.aar6759.
Vadrevu, S.K., Trbojevic-Akmacic, I., Abdel-Mohsen, M., et al. "Frontline Science: Plasma and immunoglobulin G galactosylation associate with HIV persistence during antiretroviral therapy." J Leukoc Biol. 2018 Apr 6. doi: 10.1002/JLB.3HI1217-500R.
Krarup, A.R., Abdel-Mohsen, M., Denton P.W., et al. "The TLR9 agonist MGN1703 triggers a potent type I interferon response in the sigmoid colon." Mucosal Immunol. 2018 Mar;11(2):449-461. doi: 10.1038/mi.2017.59. Epub 2017 Aug 2.
Abdel-Mohsen, M., Chavez, L., Pillai, S.K., et al. "Human Galectin-9 Is a Potent Mediator of HIV Transcription and Reactivation." PLoS Pathog. 2016 Jun 2;12(6):e1005677. doi: 10.1371/journal.ppat.1005677. eCollection 2016 Jun.
Abdel-Mohsen, M., Wang, C., Pillai, S.K., et al. "Select host restriction factors are associated with HIV persistence during antiretroviral therapy." AIDS. 2015 Feb 20;29(4):411-20. doi: 10.1097/QAD.0000000000000572.