Lab In The News
$12.5 Million Grant Awarded to Wistar Extends Federal Support of the Institute’s Quest for Improved Targeted Therapies for Melanoma
The Wistar Institute and collaborating institutions have received a major grant from the National Institutes of Health to further research on new melanoma targeted therapies integrating the role of the tumor microenvironment in influencing response to therapy and development of resistance.
How Philadelphia health care institutions will use $50M in new grants and gifts
October has proved financially rewarding for four Philadelphia health care institutions that have recently taken in a total of more than $50 million in federal government grants and donations to fund a variety of research and treatment programs.
The Herlyn Laboratory
The Herlyn laboratory at The Wistar Institute focuses on the biology that underlies melanoma. His efforts have pioneered the use of the three-dimensional “artificial skin” cultures to study the behavior of both tumor and normal cells that sustain tumor growth, a system known as the tumor microenvironment. The Herlyn Laboratory has transformed the scientific understanding of stem cells as they relate to cancer, and their work on the networks of signaling pathways in melanoma has formed the basis of numerous therapies now in clinical trials or very recently approved.
Haiyin Li, Ph.D.
Associate Staff Scientist
Eric Ramirez-Salazar, D.Sc.
Yeqing Chen, M.S.
Ling Li, MD
Gregory Schuyler Brown
Due to the high demand for our cell lines, the Herlyn lab is pleased to partner with Rockland Immunochemicals Inc. for distribution.
Please contact Rockland directly about acquiring our melanoma cell lines by contacting Rockland customer service for help with lines not found yet on their website.
For help selecting lines, see the mutational chart below or search using Rockland's website. For any questions, concerns, help or any other issues, please email Min Xiao at Wistar or contact customer service at Rockland.
Cell authentication has received considerable attention recently as more and more reports of cell line cross contaminations and misidentifications have come to light. As such, in 2008, we implemented and routinely perform Short tandem repeat (STR) profiling using AmpFlSTR® Identifiler® PCR Amplification Kit (Catalog Number 4322288) by Life Technologies which uses loci consistent with all major worldwide STR standards. PCR amplification and STR allele separation and sizing is performed by the Wistar Genomics Facility. Profile interpretation is performed in the Herlyn lab by interrogating the resulting DNA fingerprint to our internal database which includes over 1000 fingerprints, primarily Wistar Melanoma but also commonly used cell lines such as HeLa and 293T cells. The STR profile is provided here as a reference comparison to your results.
For additional inquiries, please email Min Xiao.
For inquiries regarding any of the following techniques or resourses, please email Jessica Kohn to be put in touch with the appropriate lab representative.
The Herlyn Laboratory seeks to further define the various signaling pathways that work in cancer cells in order to discover new opportunities to inhibit cancer growth through targeted therapeutics. Since therapy is increasingly guided by the genetic aberrations in tumors, Herlyn and his colleagues are developing combinations of compounds that take into account the genetic signature of tumors, with the specific goal of individualized cancer therapy. Currently, the Herlyn Laboratory collaborates with pharmaceutical companies as well as academic chemists and structural biologists to select and further develop compounds for tumor inhibition. Tumor heterogeneity, i.e., the differences between cells within one tumor, among different tumor lesions of the same patient, or between patients even if the tumors are of similar genetic signatures, provides major challenges for future therapy. The laboratory is developing biological signatures of melanoma cells that take into account the various forms of heterogeneity.
Another major effort of the Herlyn Laboratory is the study of therapy resistance and tumor dormancy. Tumor cells can become dormant in primary tumors or at any time after metastatic dissemination and can persist in the dormant state for many years, allowing tumors to resist treatment. Herlyn’s working hypothesis is that defined tumor subpopulations are central to dormancy and drug resistance due to their slow turnover and their non-responsiveness to growth signals. His efforts seek to define how tumor cells escape dormancy for growth, invasion, and metastasis, and how to best develop strategies for therapy.
Modeling the normal and diseased human tissue microenvironment
The Herlyn lab is differentiating multi-potent stem cells from the human dermis and reprogrammed stem cells into melanocytes to test the hypothesis that melanocyte stem cells are more prone to transformation than fully differentiated cells, and that neighboring cells and matrix in the microenvironment play critical roles in differentiation and transformation. The lab has developed a complex, three-dimensional model that mimics human skin, and are using it to reconstruct each step in the melanoma development and progression cascade. Genes associated with melanoma are overexpressed or silenced with shRNA constructs in lentiviral vectors and the lab increasingly uses cDNA and sh (short hairpin) RNA libraries for our experiments. Ultraviolet light irradiation is mimicking the DNA damaging effect of sunlight. Skin reconstructs can also be grafted onto immunodeficienct mice for long-term observation. Besides isolating melanocytes and keratinocytes from skin, we have begun to differentiate them from ‘induced pluripotent stem’ (iPS). This source also allows the lab to generate an intact human inflammatory and immune system in vivo, including from melanoma patients where we have cell lines or patient-derived xenografts (PDX). Studies on interactions among tumor cells, fibroblasts and endothelial cells are also done in 3-D models, in which cells are embedded into collagen to mimic the tumor microenvironment. Growing cells in organ-like models induces major changes in gene expression similar to those in animals and patients, making such models superbly suited for studies of cell-cell signaling, matrix formation, and drug resistance.
Therapeutic targeting of signaling pathways in cancer
The Herlyn lab is defining signal transduction pathways that are constitutively activated in melanoma and squamous cell cancer cells through autocrine and paracrine growth factors and genetic alterations. With shRNA in lentiviral vectors, the lab is identifying genes in tumor cells, stromal fibroblasts, and endothelial cells that are potential targets for therapy. In melanoma, the MAPK and PI3K pathways are primary targets for therapy, but other pathways are also explored for inhibition by small molecule compounds. Since therapy is increasingly guided by genetic aberrations in tumors, the lab is developing combinations of compounds that take into account the genetic abnormalities of tumors, with the long-term goal of individualized cancer therapy. In recent years, the lab has actively collaborated with pharmaceutical companies to obtain compounds in early stages of preclinical and clinical development. Increasingly, the lab is collaborating with academic chemists and structural biologists to select and further develop compounds for tumor inhibition.
Tumor dormancy and therapy resistance
Tumor cells can become dormant in primary tumors or at any time after metastatic dissemination and can persist in the dormant state for many years, allowing them to resist treatment. The working hypothesis of the Herlyn lab is that tumor-maintenance cells (tumor stem cells) are central to dormancy due to their non-proliferation or very slow turnover and their non-responsiveness to growth signals. The lab is delineating tumor dormancy in melanoma and characterizing subpopulations of cells with a major focus on slow-proliferating cells that have high proliferation potential hypothesizing that these cells are critical for dormancy and therapy resistance. The lab will then define how tumor cells escape dormancy for growth, invasion, and metastasis, and developing strategies for therapy. Using the lab's unique 3-D melanoma, Herlyn and his lab will determine how microenvironmental cues from the matrix or other cells such as B cells, macrophages, and endothelial cells drive gene activation, leading to a signaling cascade for proliferation and invasion. These studies will lead to in-depth investigations of tumor heterogeneity and the dynamic regulation of genes that define subpopulations with specialized biologic functions. The long-term goal is to develop strategies for two therapies, one for eliminating the bulk of the tumor, the other for small subpopulations that escape all major therapeutic strategies. Such combinations should achieve elimination of all tumor cells, which is required in melanoma because single tumor cells are capable of tumor induction in immunodeficient animals.
Stem cells and melanoma
Multipotent stem cells with neural crest-like properties have been identified by our lab and others in the dermis of human skin. The stem cells display self-renewal capacity and differentiate into neural crest derivatives including epidermal pigment-producing melanocytes. Neural crest-like stem cells (NCLSC) share many properties with aggressive melanoma cells, such as high migratory capabilities and expression of neural crest markers. However, little is known about which intrinsic or extrinsic signals determine proliferation or differentiation of stem cells. In our studies we have focused on major developmental pathways. Notch signaling is highly activated in stem cells, similar to cells within melanoma spheres. Inhibition of Notch signaling reduces proliferation of stem cells, induces cell death, and down-regulates non-canonical Wnt5a, suggesting that the Notch pathway contributes to maintenance and motility of the stem cells. In 3-D skin reconstructs, canonical Wnt signaling promotes differentiation of stem cells into melanocytes. This differentiation is triggered by the endogenous Notch inhibitor Numb, which is upregulated in the stem cells by Wnt7a derived from UV-irradiated keratinocytes. These studies reveal a crosstalk between the two conserved developmental pathways in human skin, and highlight the role of the skin microenvironment in driving the generation of stem cells, and possibly tumor-initiating cells. They also provide a rationale for identifying novel targets for therapy among those groups of genes that are intimately involved in melanocyte development and highly expressed in melanoma while being largely absent in normal melanocytes.
The Herlyn laboratory has a long history of collaborations with members of the Penn/Wistar campus, particularly those who have had an interest in melanoma. Additionally, the lab has partnered with several outreach organizations around the world to support melanoma research. Learn more about these collaborations.
The Herlyn laboratory is supported by a variety of grants to study melanoma. Learn more about the grants supporting this research.
The Herlyn laboratory can provide access to several additional resources. To request a copy of a research paper published by the Herlyn laboratory, or for additional lab resources, contact:
Zhang, G., Wu, L.W., Mender, I., Barzily-Rokni, M., Hammond, M.R., Ope, O., Cheng, C., Vasilopoulos, T., Randell, S., Sadek, N., et al. "Induction of Telomere Dysfunction Prolongs Disease Control of Therapy-Resistant Melanoma." Clin Cancer Res. 2018 Mar 21. pii: clincanres.2773.2017. doi: 10.1158/1078-0432.CCR-17-2773. [Epub ahead of print]
Perego, M., Maurer, M., Wang, J.X., Shaffer, S., Müller, A.C., Parapatics, K., Li, L., Hristova, D., Shin, S., Keeney, F., et al. "A slow-cycling subpopulation of melanoma cells with highly invasive properties." Oncogene. 2018 Jan 18;37(3):302-312. doi: 10.1038/onc.2017.341. Epub 2017 Sep 18.
Heppt, M.V., Wang, J.X., Hristova, D.M., Wei, Z., Li, L., Evans, B., Beqiri, M., Zaman, S., Zhang, J., Irmler, M., et al. "MSX1-Induced Neural Crest-Like Reprogramming Promotes Melanoma Progression." J Invest Dermatol. 2018 Jan;138(1):141-149. doi: 10.1016/j.jid.2017.05.038. Epub 2017 Sep 18.
Krepler, C., Sproesser, K., Brafford, P., Beqiri, M., Garman, B., Xiao, M., Shannan, B., Watters, A., Perego, M., Zhang, G., et al. "A Comprehensive Patient-Derived Xenograft Collection Representing the Heterogeneity of Melanoma." Cell Rep. 2017 Nov 14;21(7):1953-1967. doi: 10.1016/j.celrep.2017.10.021.
Lu, H., Liu, S., Zhang, G., Bin, Wu., Zhu, Y., Frederick, D.T., Hu, Y., Zhong, W., Randell, S., Sadek, N., et al. "PAK signalling drives acquired drug resistance to MAPK inhibitors in BRAF-mutant melanomas." Nature. 2017 Oct 5;550(7674):133-136. doi: 10.1038/nature24040. Epub 2017 Sep 27.
Alessandro Gardini, Ph.D.
Assistant Professor, Gene Expression & Regulation Program, The Wistar Institute Cancer Center