The Dotiwala Laboratory
The Dotiwala laboratory uses cellular, molecular, biochemical and omics approaches, as well as sophisticated in vivo imaging, to dissect the mechanism by which cell-based immunity (neutrophils, macrophages and killer lymphocytes) is activated in the presence of different pathogenic bacteria, such as intra- and extracellular bacteria, fungi and protozoa. In particular, they investigate how killer immune cells use pore forming protein perforin, antimicrobial peptide granulysin, and immune protease granzyme B in synergy to destroy pathogens in host cells. The ultimate goal of this research is to adapt different immune proteases coupled with antimicrobial peptides as therapies for drug-resistant bacterial infections. The lab is also interested in delivering immune proteases to cancer cells to restrict their ability to develop resistance.
Sachin Singh, Ph.D.