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David Speicher, Ph.D.

David W. Speicher, Ph.D.


The Speicher Laboratory


Professor and Program Co-Leader, Molecular & Cellular Oncogenesis Program, Ellen and Ronald Caplan Cancer Center

Director, Center for Systems & Computational Biology

Member, The Wistar Institute Melanoma Research Center

Scientific Director, Proteomics & Metabolomics Facility

About the Scientist

Speicher is a recognized leader in the field of biomolecular analyses using mass spectrometry approaches, including proteomics and metabolomics, which allow the systematic study of the full set of proteins and metabolites, respectively, that are produced by a given cell, tissue, or organism.

Speicher joined Wistar in 1986 from the Yale University School of Medicine. He was born and raised in Pennsylvania and attended Pennsylvania State University as both an undergraduate and graduate biochemistry student.  He received his Ph.D. in 1977 and subsequently pursued postdoctoral training at the Yale University School of Medicine.  He then accepted a position on the Yale Medical School research faculty with a joint appointment as the Director of Yale’s Protein Chemistry Laboratory, prior to moving to Wistar.

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The Speicher Laboratory

Using state-of-the-art high-resolution mass spectrometry with related experimental and computational methods, the Speicher laboratory investigates protein, polar metabolite and lipid changes associated with cancer and other clinical disorders.


Research Assistants

Lynn Beer
Sandra Harper
Peter Hembach


Learn more about current research in the Speicher laboratory

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The Speicher laboratory is pursuing three major projects as well as a number of collaborative projects in diverse biomedical research areas. Most studies utilize proteomics, lipidomics and metabolomics using mass spectrometry as major tools.


Selected Publications

Singha, B., Harper, S.L., Goldman, A.R., Bitler, B.G., Aird, K.M., Borowsky, M.E., Cadungog, M.G., Liu, Q., Zhang, R., Jean, S., Drapkin, R., Speicher, D.W., et al. “CLIC1 and CLIC4 complement CA125 as a diagnostic biomarker panel for all subtypes of epithelial ovarian cancer.” Sci Rep. 2018 Oct 3;8(1):14725. doi: 10.1038/s41598-018-32885-2.

Rebecca, V.W., Nicastri, M.C., Fennelly, C., Chude, C.I., Barber-Rotenberg, J.S., Ronghe, A., McAfee, Q., McLaughlin, N.P., Zhang, G., Goldman, A.R., et al. “PPT1 Promotes Tumor Growth and Is the Molecular Target of Chloroquine Derivatives in Cancer.” Cancer Discov. 2019 Feb;9(2):220-229. doi: 10.1158/2159-8290.CD-18-0706. Epub 2018 Nov 15.

Goldman, A.,Beer, L., Tang, H., Hembach, P., Zayas-Bazan, D., Speicher, D. “Proteome Analysis Using Gel-LC-MS/MSCurr Protoc Protein Sci. 2019 Jun;96(1):e93. doi: 10.1002/cpps.93. Epub 2019 Jun 10.

Alicea, G.M., Rebecca, V.W., Goldman, A.R., Fane, M.E., Douglass, S.M., Behera, R., Webster, M.R., Kugel, C.H. 3rd, Ecker, B.L., Caino, M.C., et al. “Changes in Aged Fibroblast Lipid Metabolism Induce Age-Dependent Melanoma Cell Resistance to Targeted Therapy via the Fatty Acid Transporter FATP2.” Cancer Discov. 2020 Sep;10(9):1282-1295. doi: 10.1158/2159-8290.CD-20-0329. Epub 2020 Jun 4.

Li, J., Agarwal, E., Bertolini, I., Seo, J.H., Caino, M.C., Ghosh, J.C., Kossenkov, A.V., Liu, Q., Tang, H.Y., Goldman, A.R., et al. “The Mitophagy Effector FUNDC1 Controls Mitochondrial Reprogramming And Cellular Plasticity In Cancer Cells.” Sci Signal. 2020 Jul 28;13(642):eaaz8240. doi: 10.1126/scisignal.aaz8240.

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