Introduction to Imaging Cytometry

The Amnis ImageStreamX imaging flow cytometer combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy.

Microscopy offers detailed cellular images and morphologic information, which are useful scientific tools for the study of cell function.  However, the interpretation of microscopic imagery can be subjective, qualitative, and laborious.  Flow cytometry is excellent for quantitative phenotyping and yields statistically robust results by rapidly interrogating large numbers of cells. However, flow cytometry lacks any ability to image, so sub-cellular localization and cell function are measured indirectly.   The Amnis ImageStreamX produces multiple high resolution images of every cell directly in flow, including brightfield, darkfield (SSC) and up to 5 fluorescent markers.  By combining the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insight of microscopy, imaging cytometry opens the door to an extensive range of novel applications.

Common imaging cytometry applications

Cell Signaling:  NFkB Translocation, HIV induced NFAT, FoxP3 localization

Co-localization: T cell / APC (immune synapse), capping / surface co-localization

Cell-Cell Interactions: Immune synapse formation, NFkB activation from T-cell APC conjugation

Morphology: MCP-1 activation of monocytes, differentiation of FDCP cells

Internalization: CpGB, Internalization, phagocytosis of bacteria by monocytes

Cell Death, DNA Damage and Repair, Cell Cycle and Mitosis:  Apoptosis, nuclear fragmentation, caspase3 activation