User Fees and Access

All customers should register for an iLab account prior to the initiation of a new project. To register for an account go to https://wistar.corefacilities.org/account/login. Customer billing information and a valid 10 digit grant number (Wistar members only) or PO# (all external users) must be provided prior to the initiation of any work. First-time external users are required to provide their business administrator’s name, address, telephone number, fax number, and email address. For assistance contact the facility staff at molecular_screening@wistar.org.

All work requests are initiated on a first-come, first-served basis, with Wistar Cancer Center members receiving priority and all other user requests being fulfilled as soon as possible.

Fees may be charged at the start of a specific job for any services offered.

Please acknowledge the facility if the materials generated by the Facility are used to create publication data.

Facility services are divided into two areas, Molecular Screening and Protein Expression. For additional information about user fees and access click on a link below: 

Molecular Screening Service User Fees and Access
Protein Expression Service User Fees and Access

Molecular Screening User Fees and Access

The cost of a screen is dependent upon the specifics of the assay developed.  Generally, the cost per a data point can range from $0.10 to $5, depending upon the endpoint readout. The direct cost of a screen is determined on the basis of four components: consumables, personnel time, machine time, and library.

The laboratory provides modular pricing schedules designed to keep the facility’s services as accessible as possible to all investigators. Once assay conditions have been optimized and tested, the exact cost of a screen will be determined.  Depending on the complexity of an assay/screen, implementation may be divided into incremental milestones along with cost estimates.  Customers may decide scope of screen including: the library and number of compounds, number of replicates, analyses and follow-ups.

How to initiate a screening project:
A high-throughput screening project is initiated by completion of an application, followed by extensive consultation between the Managing Director, the Scientific Director and the initiating Cancer Center member.  The goals of the application and this initial consultation are to define the scope of the project, assess its feasibility, define responsibilities, and set preliminary expectations.  Additional applications must be submitted each time a new screen is proposed.

 

Protein Expression User Fees and Access

User Fees

Rates are established based on a yearly assessment of fixed and variable costs associated with providing a given service. All costs are annually updated to reflect the budgeted operating costs of the Facility.   

All users are encouraged to email protein_expression@wistar.org to discuss the scope of the project, available services, and desired deliverables in order to quote appropriate service fees.

Access

The PI and/or lab investigator supplying a plasmid or viral expression vector to the Facility is responsible for providing correct (verified) plasmid constructs and gene sequences for expression. The Facility is responsible for verifying any constructs that are produced within the facility, by facility staff, and for performing services correctly.

Once a service is initiated, there will be no refunds even if canceled. It is at the discretion of the facility director whether a discount on a subsequent service will be offered. The amount of the discount will be prorated based on the actual labor and expenses spent on the project before cancellation.

Due to the uncertainties associated with volume of work and cell culture, the time frames provided for the various services are for perspective only.  Although we make every effort to perform the services within the given time frames, we cannot guarantee them based on current workloads.

Due to the intrinsic biochemical properties of each protein, we do not guarantee recombinant protein solubility, purity, or yield. We will provide details of all expression trials, purification strategies, and provide consultation regarding alternative strategies or approaches to solve your protein expression needs.

Protein preparation services are ordered based on the cell culture volume.

Due to variability in packaging of different retroviral vectors, we do not guarantee the production of a minimum titer.  We recommend, whenever possible that newly produced vectors are titrated.

It is the responsibility of the investigator to register the intended specific use of recombinant DNA technology (i.e. retrovirus use) with Wistar’s Institutional Biosafety Committee (IBC) as required by the most current NIH guidelines

DNA plasmids, bacterial stocks, cell pellets, or virus stocks can be shipped or brought directly to the Facility.

For shipments, please send samples to:

The Wistar Institute
Molecular Screening and Protein Expression Facility, Room 352
3601 Spruce Street
Philadelphia, PA 19104

Tel: 215-898-3814