Useful Information

shRNA Ordering Guidelines

NCGC assay Guidance manual

General Assay development
High-throughput screening assays for the identification of chemical probes. Inglese J, Johnson RL, Simeonov A, Xia M, Zheng W, Austin CP, Auld DS. Nat Chem Biol. 2007 Aug;3(8):466-79. PMID: 17637779

Luciferase based screens
Characterization of chemical libraries for luciferase inhibitory activity.Auld DS, Southall NT, Jadhav A, Johnson RL, Diller DJ, Simeonov A, Austin CP, Inglese J. J Med Chem. 2008 Apr 24;51(8):2372-86. Epub 2008 Mar 26. PMID: 18363348

Fluoresence: Intensity, anisotropy/polarization (FA/FP)
Homogeneous fluorescence readouts for miniaturized high-throughput screening: theory and practice. Pope AJ, Haupts UM, Moore KJ. Drug Discov Today. 1999 Aug;4(8):350-362. PMID: 10431145

Fluorescence polarization and anisotropy in high throughput screening: perspectives and primer. Owicki JC. J Biomol Screen. 2000 Oct;5(5):297-306. PMID: 11080688

Real experiences of uHTS: a prototypic 1536-well fluorescence anisotropy-based uHTS screen and application of well-level quality control procedures. Turconi S, Shea K, Ashman S, Fantom K, Earnshaw DL, Bingham RP, Haupts UM, Brown MJ, Pope AJ. J Biomol Screen. 2001 Oct;6(5):275-90. PMID: 11689128

Aggregation based inhibition
A high-throughput screen for aggregation-based inhibition in a large compound library. Feng BY, Simeonov A, Jadhav A, Babaoglu K, Inglese J, Shoichet BK, Austin CP. J Med Chem. 2007 May 17;50(10):2385-90. PMID: 17447748

Thermal stability
Evaluation of fluorescence-based thermal shift assays for hit identification in drug discovery. Lo MC, Aulabaugh A, Jin G, Cowling R, Bard J, Malamas M, Ellestad G. Anal Biochem. 2004 Sep 1;332(1):153-9. PMID: 15301960

Data analysis/reporting
A simple statistical parameter for use in evaluation and validation of high throughput screening assays. Ji-Hu Zhang, Thomas D. Y. Chung and Kevin R. Oldenburg (1999). J. Biomol. Screen 4:67-73.

Statistical practice in high-throughput screening data analysis. Malo N, Hanley JA, Cerquozzi S, Pelletier J, Nadon R. Nat Biotechnol. 2006 Feb;24(2):167-75. PMID: 16465162

Reporting data from high-throughput screening of small-molecule libraries. Inglese J, Shamu CE, Guy RK. Nat Chem Biol. 2007 Aug;3(8):438-41. PMID: 17637769