Imaging Facility

Overview

The imaging of complex cellular structures is used to determine how the temporal and spatial organization of regulatory events within cells, tissues and organisms impacts both normal and pathological processes. The state-of-the-art Imaging Shared Resource provides access to standard and advanced optical imaging systems capable of reaching these goals and offers assistance with advanced image analysis solutions. Researchers may be trained for unassisted use of all core instrumentation, while full service assistance by facility staff is also available for qualitative or quantitative image capture. The Facility also offers expert technical assistance with experimental design to optimize imaging results, enabling users to get more out of the imaging technology.

Services

  • Widefield microscopy
  • Confocal microscopy
  • 2-photon intravital microscopy
  • Advanced quantitative imaging (FRET, FRAP, photobleaching, colocalization)
  • 2D, 3D, 4D, 5D and 6D live-cell timelapse imaging
  • Small animal, whole body luminescence, fluorescence, and CT imaging
  • Low magnification and photomacrography
  • Personal training and assistance on all imaging systems
  • Image analysis training and services
  • Automated and facilitated whole-slide scanning
  • Individual assistance with microscopes within Wistar labs
  • Traditional photographic services

Equipment & Features

  • Upright widefield microscopes (brightfield and fluorescence)
    • Nikon 80i upright microscope
    • Nikon E600 upright microscope
    • Zeiss Axioskop2 upright microscope
    • Nikon SMZ800 and 1500 stereomicroscopes
  • Inverted widefield microscopes (brightfield and fluorescence)
    • Nikon TE2000 inverted microscope
    • Nikon TE300 inverted microscope (with incubation chamber)
    • Fully Automated Nikon Eclipse Ti2 inverted microscope
  • Laser-based instruments (for fixed and live-cell imaging)
    • Leica TCS SP5 II laser scanning spectral confocal microscope
    • Leica TCS SP8 X WLL laser scanning spectral confocal microscope
    • Leica TCS SP8 MP 2-photon intravital microscope
    • Leica Spinning Disk confocal microscope
  • Other imaging systems
    • IVIS SpectrumCT small animal, whole body imaging system
    • Digital SLR camera, copy stand and portable lighting
    • Advanced image analysis workstations

This facility is supported in part by a Cancer Center Support Grant (CCSG) awarded by the National Cancer Institute (NCI) to The Wistar Institute Cancer Center.

Contact Us

The Wistar Institute
Imaging Facility, Room 287
3601 Spruce Street
Philadelphia, PA 19104

Phone: 215-898-3887
Fax: 215-898-3868

Facility Hours:
8:30 a.m.-5:00 p.m., Monday-Friday

Qing Chen, M.D., Ph.D.
Scientific Director

James Hayden, B.A., R.B.P., F.B.C.A.
Managing Director
215-898-3887

Frederick Keeney, B.S.
Wistar Research Assistant
215-898-3887

Aubrey Leso, B.S.
Research Assistant I
215-898-3887