Histotechnology Facility

Overview

The Histotechnology Shared Resource provides services for fixing, processing and paraffin or OCT-embedding of all types of tissues for light microscopy (e.g. routine stains, immunohistochemistry or in situ hybridization). The Facility staff performs routine hematoxylin and eosin staining, as well as specialized staining and slide preparation for immunohistochemistry and in situ hybridization. Frozen sectioning is also available, including consultation regarding freezing and fixing techniques to optimize experimental results.

Services

  • Processing, embedding, sectioning, and histochemical staining of specimens
  • Paraffin and frozen sectioning are routinely available for light microscopy and in situ hybridization
  • Tissue preparation for DNA/RNA extraction
  • Various histochemical staining procedures are performed, including those required for neuropathology
  • Special procedures and staining protocols
  • Consultation regarding freezing and fixing techniques and immunohistochemistry staining techniques

Equipment

  • Tissue-Tek VIP processor
  • Zeiss HMS slide stainer
  • Leica CV-5000 coverslipping machine
  • Hacker RCM-3655 coverslipping machine
  • TissueTek embedding center
  • Reichert-Jung 2065 rotary microtome
  • Shandon cryostat E
  • Thermo HM355S Automatic Rotary Microtome

Contact Us

The Wistar Institute
Histotechnology Facility
3601 Spruce Street, Room 258
Philadelphia, PA 19104

Phone: 215-898-0027
Fax: 215-898-3868

Facility Hours:
9:00 a.m.-5:00 p.m., Monday-Friday
[Sample submission and pickup are available 24 hours a day, 7 days a week.]

Fangping Chen, HT (ASCP)
Managing Director

Maureen Murphy, Ph.D.
Scientific Director

 

Services

Learn more about Histotechnology Facility's Services... Click Here >