The Histotechnology Shared Resource provides services for fixing, processing and paraffin or OCT-embedding of all types of tissues for light microscopy (e.g. routine stains, immunohistochemistry or in situ hybridization). The Facility staff performs routine hematoxylin, eosin staining, immunochemistry and FISH staining, as well as specialized staining and slide preparation for immunohistochemistry and in situ hybridization. Frozen sectioning is also available, including consultation regarding freezing and fixing techniques to optimize experimental results.
Services
Processing, embedding, sectioning, and histochemical staining of specimens
Paraffin and frozen sectioning are routinely available for light microscopy and in situ hybridization
Tissue preparation for DNA/RNA extraction
Various histochemical staining procedures are performed, including those required for neuropathology
Special procedures and staining protocols
Consultation regarding freezing and fixing techniques and immunohistochemistry staining techniques
Equipment
Thermo cryostar NX50
Thermo Excelsior AS tissue processor
Dakewe auto stainer
Dakewe coverslipping machine
TissueTek embedding center
Thermo HM355S microtome
Biocare Intellipath IHC stainer
Pricing
For pricing information, visit iLab or contact the managing director.
National Institutes of Health (NIH) has introduced new standards for grant applications to address an increasing trend of reports of failure to replicate important basic/preclinical studies.
Contact Us
The Wistar Institute Histotechnology Facility Room 258 3601 Spruce Street Philadelphia, PA 19104 215-898-0027
Directors
Daniel Claiborne, Ph.D. Scientific Director of the Histotechnology Facility
Fangping Chen, HT (ASCP) Managing Director
Facility Hours
9:00 a.m. – 5:00 p.m. Monday-Friday
[Contact the facility to make arrangements for sample submission and pickup.]
