Monoclonal Antibody A32 Against MEL-CAM/MUC18
Hybridoma cells producing MAb A32 were obtained after fusion of the murine myeloma cell line P3X63Ag8.653 with spleen cells derived from BALB/c mice which were immunized by i.p. injection of 1.5 x 107 human primary WM902B melanoma cells and boosted with the same treatment procedures 2 and 7 weeks later.
Cell surface melanoma-associated antigens can mediate cell-cell or cell-substrate adhesion, signal transduction, proteolysis, or immune recognition and play a key role in determining invasive and metastatic competence of the tumor cells. MAb A32 binds to endothelial and melanoma cells and detects a 113 Kd cell surface adhesion receptor of the immunoglobulin supergene family. The Mel-CAM/MUC18 antigen is a cell surface molecule involved in heterotypic adhesion. The ligand for Mel-CAM/MUC18 has not yet been identified. Its characterization will be important for endothelial cell adhesion during inflammation.
immunohistochemistry, western blot, endothelial cell identification (demonstrating strong staining of all endothelial cells), frozen tissue sections, melanomas.