Monoclonal Antibodies That Bind to Cell Surface Antigens on Human Embryonal Carcinoma Cells
Barbara Knowles, Davor Solter, and Peter Andrews
MC-631 (SSEA-3) hybridomas were produced by fusing SP2/0 mouse myeloma cells with splenocytes from a Fisher rat immunized with 4-8 cell stage mouse embryos.
MC-813-70 (SSEA-4) hybridomas were produced by fusing SP2/0 myeloma cells with splenocytes from a BALB/c mouse immunized with human embryonal carcinoma cell line 2102Ep.
TRA-1-60R and TRA-1-81 hybridomas were produced by fusing SP2/0 myeloma cells with splenocytes from a BALB/c mouse immunized with human embryonal carcinoma cell line 2102Ep.
MC-631 (SSEA-3) and MC813-70 (SSEA-4): early mouse embryos, human teratocarcinoma stem cells, human embryonal stem cells. MC-631, IgM; MC-813-70, IgG3
TRA-1-60R and TRA-1-81: human embryonal carcinoma cell lines. IgM.
Immunohistochemistry, Immunoprecipitation. Identification of human embryonic stem cells. Characterization of embryonal stem cells of human origin to distinguish them from those of murine origin.
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