BALB/c mice were immunized with the human recombinant bFGF fusion protein from E.coli. Fusion of spleen cells of immunized mice was done with the non-secreting mouse myeloma cell line P3x63Ag8.SP2/0.

Both antibodies bind to the recombinant and native forms of bFGF. They bind the protein by Western Blotting. They also bind to cells expressing bFGF in frozen tissue sections. In normal skin, there is little binding of the antibodies but both bind strongly to nevi and melanomas.

Western blotting, Immunohistochemistry, ELISA