BAP1 mAbs are secreted by hybridomas derived from mice immunized with full-length BAP1. This protein was expressed and purified from E.coli. Individual mAbs were selected for reactivity to various domains of BAP1 fused to GST. Mab 1G8 and Mab 2A2 recognize epitopes within the UCH domain of BAP1. Mab 3C11 recognizes an epitope within the region of amino acids 337-440.

All mAbs recognize full-length BAP1 produced by 1) in vitro transcription/translation; 2) over-expression in transiently transfected COS1 cells; and 3) over-expression from stably transfected MCF7 cells {breast cancer cell line} and from stably transfected NCI-H226 cells (non-small cell lung carcinoma cell line).

These antibodies have been used in immunoprecipitation and immunofluorescense analyses.