Protein Expression and Libraries Facility

Instrumentation (Rooms G68, G70 and G72)

Rooms G68, G70 and G72 are equipped for cell culture and molecular
biology with:

• Zeiss Axiovert 10 phase-contrast microscope,
• 2 class II type laminar flow hoods,
• 3 x 25 cu. ft upright incubators (27°C) with multi-position magnetic stirrers designated for insect cell culturing and baculovirus infection
  of insect cells in spinner flasks, 
• 2 dual-chamber CO2 incubators,
• Sorval legend Mach1.6R and Beckman CS-6R low speed bench-top centrifuge
• benchtop Innova 4000 temperature controlled orbital shaker,
• floor model ThermoForma refrigerated environmental shaker with capacity  for 12 x 2L flasks,
• AKTA purifier FPLC,
• Branson 450 sonnicator,
• 3 refrigerators, 1 cold box, 2 x -20°C freezers, one -40°C freezer, 3 x -80°C freezers,
• Milli-Q water purification system,

The Facility is also well equipped with standard laboratory equipment, including micro-centrifuges, PCR machines, water baths, microwave, gel electrophoresis units, balances, pH meter, conductivity meter, and standard supplies for conventional and affinity chromatography approaches to purify recombinant proteins from either bacteria or insect cells.  In addition, the Facility has access to an adjacent common use equipment room (G69) that contains a Molecular Dynamics FluorImager SI, Storm 820 phosphoimager, Typhoon imager, a Sorvall RC-5B high speed centrifuge and rotors.