User Fees

Labor charges are $50 per hour for Wistar Investigators, $60 per hour for non-Wistar academic users, and $80.00 per hour for commercial users.

*There is a 20% surcharge for Non-Wistar Academic users and a 55% surcharge for commercial users on all facility fees.

Please note that the fees for Bioinformatics are separate.

Jump to the Illumina Genome Analyzer services charges.

Description

Type

Wistar Investigator*

Illumina whole-genome expression for Human and mouse total RNA to be amplified (Ambion or Epicentre kits), hybridized, washed & scanned

6 Sample
Beadchip

$1,920/chip
($320/sample)

Human HT-12 beadchip

$2,820/chip
($235/sample)

Illumina whole-genome expression for Human and mouse total RNA to be amplified and labeled (NuGEN Kits), hybridized, washed & scanned

6 Sample
Beadchip

$2,130/chip
($355/sample)

Human HT-12 beadchip

$3,240/chip
($270/sample)

SNP Genotyping Analysis

mouse low density (Ld) linkage panel, 96 samples

Upon request

Illumina DASL Gene Expression for degraded RNA samples with RNA isolation

Whole Genome DASL Assay,
24 samples**

$275/sample

Illumina DASL Gene Expression for degraded RNA samples without RNA isolation

Whole Genome DASL Assay,
24 samples**

$239/sample

Illumina Infinium Whole-genome SNP genotyping and Copy Number Variation (CNV) Analysis

Upon request

Upon request

Illumina Infinium Methylation profiling with Bisulfite conversion

Upon request

Upon request

DNA Bisulfite conversion

High Pure RNA Paraffin Kit

$25/sample

DNA isolation

Tri-reagent

$30/sample

RNA isolation

Tri-reagent or RNAqueous-Micro kit

$30/sample

RNA isolation

Paxgene kit

$36/sample

RNA isolations ribbons

High Pure RNA Paraffin kit

$36/sample

RNA Amplification

Ambion or Epicentre

$75/sample

RNA Amplification plus labeling

NuGEN

$110/sample

ABI OpenArray miRNA assay

Human and mouse plates

$300/sample

ABI OpenArray digital, pathway specific PCR and custom miRNA and gene expression assays

Upon request

Upon request

ABI 7900 Real-time qPCR

Upon request

Upon request

ABI 3130xl Sequencing system

DNA samples

$7 per sequence

Microsatellite analysis, 5 loci

$20/sample

Microsatellite analysis, 16 loci

$40/sample

Genomic scale expression profiling requires time and an understanding of the inherent complexities of microarrays. We recommend that each investigator take time before submitting samples to discuss the project with one of our array specialists in the facility. This will help predict the number of replicates needed and estimate the time and cost of a project and improve the chances that the arrays will yield results you can have confidence in.

**Assays must include 24 samples. For less than 24 samples, please contact Celia Chang to discuss alternatives.


For Information about Illumina Genome Analyzer IIx Sequencing, please email the Solexa specialist.

Please note that the fees for Bioinformatics are separate.

Illumina Genome Analyzer

Illumina Genome Analyzer - Sample Prep + Sequencing

Wistar Investigator*

ChIP-seq sample prep + 36bp Sequencing
(+ $300 for 72bp)

$1,250

Truseq Small RNA sample prep + 36bp Sequencing
(+ $300 for 72bp)

$1,475

Genomic DNA sample prep + 36bp Sequencing
(+ $300 for 72bp)

$1,425

Truseq mRNA-seq sample prep + 36bp Sequencing
(+ $300 for 72bp)

$1,425

TruSeqChIP samples prep

$300

ScriptSeqRNA sample prep. with ribozero

$350

ScriptSeqRNA sample prep. without ribozero

$250

ScriptMiner smRNA sample prep.

$275

TruseqRNA samples prep 24 samples and more

$300

TruseqRNA samples prep 12 to 23 samples

$375

TruseqRNA samples prep <12 samples

$475

Paired-end Sample Prep + 36bp 2 Read Sequencing

$2,100

Paired-end Sample Prep + 72bp 2 Read Sequencing

$2,500

Illumina Genome Analyzer - Sequencing Only

Wistar Investigator*

36bp Sequencing only (Client prepares samples)

$950

72bp Sequencing only (Client prepares samples)

$1,250

Paired-end 36bp 2 Read Sequencing only
(Client prepares samples)

$1,550

Paired-end 72bp 2 Read Sequencing only
(Client prepares samples)

$1,950

Extra 36bp sequencing

$300

Illumina Genome Analyzer - Multiplex Sequencing

Wistar Investigator*

Additional cost Multiplexing (up to 12 samples per lane)

– Cost per flowcell

$400

Exome Sequencing

Upon request

Bioanalyzer DNA (per sample)

$28

Bioanalyzer RNA (per sample)

$18

Picogreen assay (per sample)

$18

  

Hi-Seq 2000 sequencing costs through Wistar Genomics

Other Genomics Center for Hiseq 2000

Service

Wistar Cost

Outside Academic User Cost

 

Beckman Coulter Genomics per lane

2X100

$2,200.00

$2,450.00

Beckman Coulter Genomics per lane

2x75

$2,144.00

$2,394.00

Beckman Coulter Genomics per lane

2x50

$1,744.00

$2,000.00

Beckman Coulter Genomics per lane

1x100

$1,350.00

$1,600.00

Beckman Coulter Genomics per lane

1x75

$1,299.00

$1,550.00

Beckman Coulter Genomics per lane

1x50

$1,053.00

$1,300.00

Beckman Coulter Genomics per lane

Library QC

$75.00

$75.00

BGI @CHOP per lane

2x100

$1,650.00

 

prep the correct library submission amount and shipping per sample

 

$60.00

$72.00

Please email Celia Chang if you have specific questions.