Helpful Information

FlowJo & other software
Tools for multicolor flow cytometry
Purdue website for flow cytometry
Publication attribution

FlowJo, Summit, WinMDI - Post-acquisition analysis software

Wistar has a site license for FlowJo, and the hardware addresses of individual computers are registered.  Users are billed based on a quarterly basis. If you open up and use FlowJo more than once during any given quarter, that computer will be accounted for. The Flow Cytometry Facility is sent an invoice at the end of each year, and at that time your lab will be charged. Flow cytometry files from any instrument at Wistar can be analyzed in FlowJo. Those files are uploaded onto the Wistar servers by individual users or by facility personnel at your request.  Contact the facility for instructions to register.

Summit (PC) is the free software running on the MoFlo and CyAn, and may be used to analyze data files from any of the available instruments.  Please contact Jeffrey Faust if you are interested in obtaining a copy.

WinMDI is a Windows based, free but limited post-acquisition analysis software package which may be used to analyze flow cytometry data files from any of the available instruments. Curve smoothing, re-gating of list mode data, and overlays are some of the available functions. Generated images may be cut-and-pasted into PowerPoint presentation software. Download the software.

 

Tools for multicolor flow cytometry

These give very useful variety of tools for constructing workable multicolor panels, based on lasers and fluorochrome excitation/emission spectra. Additional links give basic understanding of flow cytometry:

A suite of tools

Spectra viewers for commonly used fluorochromes from BD Biosciences

Spectra viewers for commonly used fluorochromes from Invitrogen

General flow cytometry tutorial

A quick tutorial on compensation


Purdue website for flow cytometry

The total flow cytometry nerd website provides useful links, as well as discussion groups where you can post specific questions and comments (check the “Cytometry E-mail” link).
Purdue University Cytometry Laboratories

 

Publication attribution

Publications using data generated using equipment from the facility, which require a description in a “materials/methods” or similar section should use some variation of the following:

Samples were analyzed using a BD LSRII (BD Biosciences, San Jose, CA)
Samples were analyzed using a BD FACSCalibur (BD Biosciences, San Jose, CA)
Samples were analyzed using an EPICS XL (Beckman-Coulter, Inc., Miami, FL)
Samples were analyzed using a CyAn ADP (Beckman-Coulter, Inc., Miami, FL)
Samples were sorted (or analyzed) using a FACS ARIAII (BD Biosciences, San Jose, CA)
Samples were sorted (or analyzed) using a MoFlo Legacy (Beckman-Coulter, Inc., Miami, FL)

If you are uncertain of which instrument you used, or need more technical information, please contact the facility. 

For publication purposes, the names of current Wistar Flow Cytometry personnel are:

Jeffrey S. Faust (jsfaust@wistar.org)
Christopher Corcoran (ccorcoran@wistar.org)
Scott Weiss (sweiss@wistar.org)